diff --git a/biology/p5-transdecoder/Makefile b/biology/p5-transdecoder/Makefile
index 244f4b7b8bae..f5a7bc62e9dc 100644
--- a/biology/p5-transdecoder/Makefile
+++ b/biology/p5-transdecoder/Makefile
@@ -1,9 +1,8 @@
 # $FreeBSD$
 
 PORTNAME=	transdecoder
-PORTVERSION=	2.1.0
-PORTREVISION=	1
-DISTVERSIONPREFIX=v
+DISTVERSIONPREFIX=	TransDecoder-v
+DISTVERSION=	5.4.0
 CATEGORIES=	biology perl5
 PKGNAMEPREFIX=	p5-
 
@@ -15,31 +14,35 @@ LICENSE=	BSD3CLAUSE
 RUN_DEPENDS=	cd-hit>=4.6.1:biology/cd-hit \
 		p5-URI>0:net/p5-URI
 
-NO_BUILD=	yes
-NO_ARCH=	yes
 USES=		perl5 shebangfix
+USE_GITHUB=	yes
+
+GH_PROJECT=	TransDecoder
 SHEBANG_FILES=	sample_data/cufflinks_example/runMe.sh \
 		sample_data/pasa_example/runMe.sh \
 		sample_data/simple_transcriptome_target/runMe.sh \
 		util/pfam_mpi.pbs
 
-USE_GITHUB=	yes
-GH_PROJECT=	TransDecoder
+NO_BUILD=	yes
+NO_ARCH=	yes
 
 PERL_MOD_DIR=	${PREFIX}/${SITE_PERL_REL}/${PORTNAME}
 PERL_UTIL_DIR=	${PREFIX}/libexec/${PORTNAME}
+SCRIPTS=	${WRKSRC}/TransDecoder.LongOrfs \
+		${WRKSRC}/TransDecoder.Predict
 
 post-patch:
-	${REINPLACE_CMD} \
+	@${REINPLACE_CMD} \
 		-e 's|$$FindBin::RealBin/PerlLib|${PERL_MOD_DIR}|g' \
 		-e 's|$$FindBin::Bin/../PerlLib|${PERL_MOD_DIR}|g' \
 		-e 's|$$FindBin::RealBin/util|${PERL_UTIL_DIR}|g' \
-		${WRKSRC}/TransDecoder* ${WRKSRC}/util/*.pl
+		${SCRIPTS} \
+		${WRKSRC}/util/*.pl
 
 do-install:
-	${MKDIR} ${STAGEDIR}${PERL_MOD_DIR} ${STAGEDIR}${PERL_UTIL_DIR}
+	@${MKDIR} ${STAGEDIR}${PERL_MOD_DIR} ${STAGEDIR}${PERL_UTIL_DIR}
 	${INSTALL_SCRIPT} ${WRKSRC}/PerlLib/* ${STAGEDIR}${PERL_MOD_DIR}
-	${INSTALL_SCRIPT} ${WRKSRC}/TransDecoder* ${STAGEDIR}${PREFIX}/bin
+	${INSTALL_SCRIPT} ${SCRIPTS} ${STAGEDIR}${PREFIX}/bin
 	${INSTALL_SCRIPT} ${WRKSRC}/util/*.pl ${STAGEDIR}${PERL_UTIL_DIR}
 
 .include <bsd.port.mk>
diff --git a/biology/p5-transdecoder/distinfo b/biology/p5-transdecoder/distinfo
index bf42546af4c0..fdf185b1cb55 100644
--- a/biology/p5-transdecoder/distinfo
+++ b/biology/p5-transdecoder/distinfo
@@ -1,2 +1,3 @@
-SHA256 (transdecoder-TransDecoder-v2.1.0_GH0.tar.gz) = 681a8dd72e482cb322d74d8431ddcda61f25c53ad5453d0e22c02c92b2157b24
-SIZE (transdecoder-TransDecoder-v2.1.0_GH0.tar.gz) = 11500688
+TIMESTAMP = 1540225907
+SHA256 (transdecoder-TransDecoder-TransDecoder-v5.4.0_GH0.tar.gz) = a6fa776c6eac30922632df1a5362b7564bfcb05728daeb53d0c2c3a9dc899df5
+SIZE (transdecoder-TransDecoder-TransDecoder-v5.4.0_GH0.tar.gz) = 15748557
diff --git a/biology/p5-transdecoder/pkg-descr b/biology/p5-transdecoder/pkg-descr
index 7cb7ca555079..a3ad8cc244d6 100644
--- a/biology/p5-transdecoder/pkg-descr
+++ b/biology/p5-transdecoder/pkg-descr
@@ -1,6 +1,6 @@
-TransDecoder identifies candidate coding regions within transcript
-sequences, such as those generated by de novo RNA-Seq transcript
-assembly using Trinity, or constructed based on RNA-Seq alignments
-to the genome using Tophat and Cufflinks.
+TransDecoder identifies candidate coding regions within transcript sequences,
+such as those generated by de novo RNA-Seq transcript assembly using Trinity,
+or constructed based on RNA-Seq alignments to the genome using Tophat and
+Cufflinks.
 
 WWW: http://transdecoder.github.io/
diff --git a/biology/p5-transdecoder/pkg-plist b/biology/p5-transdecoder/pkg-plist
index d394d7fa2914..8ec718231222 100644
--- a/biology/p5-transdecoder/pkg-plist
+++ b/biology/p5-transdecoder/pkg-plist
@@ -1,22 +1,23 @@
 bin/TransDecoder.LongOrfs
-bin/TransDecoder.LongOrfs.bak
 bin/TransDecoder.Predict
-bin/TransDecoder.Predict.bak
+%%SITE_PERL%%/transdecoder/DelimParser.pm
 %%SITE_PERL%%/transdecoder/Fasta_reader.pm
 %%SITE_PERL%%/transdecoder/Fasta_retriever.pm
-%%SITE_PERL%%/transdecoder/GFF3_utils.pm
+%%SITE_PERL%%/transdecoder/GFF3_utils2.pm
+%%SITE_PERL%%/transdecoder/GTF.pm
+%%SITE_PERL%%/transdecoder/GTF_utils2.pm
 %%SITE_PERL%%/transdecoder/Gene_obj.pm
-%%SITE_PERL%%/transdecoder/Gene_obj_indexer.pm
 %%SITE_PERL%%/transdecoder/Longest_orf.pm
 %%SITE_PERL%%/transdecoder/Nuc_translator.pm
-%%SITE_PERL%%/transdecoder/TiedHash.pm
+%%SITE_PERL%%/transdecoder/Overlap_piler.pm
+%%SITE_PERL%%/transdecoder/PWM.pm
+%%SITE_PERL%%/transdecoder/Pipeliner.pm
+%%SITE_PERL%%/transdecoder/Process_cmd.pm
+%%SITE_PERL%%/transdecoder/overlapping_nucs.ph
 libexec/transdecoder/cdna_alignment_orf_to_genome_orf.pl
 libexec/transdecoder/compute_base_probs.pl
-libexec/transdecoder/create_start_PSSM.pl
-libexec/transdecoder/cufflinks_gtf_genome_to_cdna_fasta.pl
-libexec/transdecoder/cufflinks_gtf_to_alignment_gff3.pl
-libexec/transdecoder/cufflinks_gtf_to_bed.pl
-libexec/transdecoder/extract_FL_subset.pl
+libexec/transdecoder/exclude_similar_proteins.pl
+libexec/transdecoder/fasta_prot_checker.pl
 libexec/transdecoder/ffindex_resume.pl
 libexec/transdecoder/gene_list_to_gff.pl
 libexec/transdecoder/get_FL_accs.pl
@@ -24,9 +25,18 @@ libexec/transdecoder/get_longest_ORF_per_transcript.pl
 libexec/transdecoder/get_top_longest_fasta_entries.pl
 libexec/transdecoder/gff3_file_to_bed.pl
 libexec/transdecoder/gff3_file_to_proteins.pl
-libexec/transdecoder/index_gff3_files_by_isoform.pl
+libexec/transdecoder/gff3_gene_to_gtf_format.pl
+libexec/transdecoder/gtf_genome_to_cdna_fasta.pl
+libexec/transdecoder/gtf_to_alignment_gff3.pl
+libexec/transdecoder/gtf_to_bed.pl
 libexec/transdecoder/nr_ORFs_gff3.pl
 libexec/transdecoder/pfam_runner.pl
+libexec/transdecoder/refine_gff3_group_iso_strip_utrs.pl
+libexec/transdecoder/refine_hexamer_scores.pl
 libexec/transdecoder/remove_eclipsed_ORFs.pl
-libexec/transdecoder/score_CDS_liklihood_all_6_frames.pl
-libexec/transdecoder/seq_n_baseprobs_to_logliklihood_vals.pl
+libexec/transdecoder/score_CDS_likelihood_all_6_frames.pl
+libexec/transdecoder/select_best_ORFs_per_transcript.pl
+libexec/transdecoder/seq_n_baseprobs_to_loglikelihood_vals.pl
+libexec/transdecoder/start_codon_refinement.pl
+libexec/transdecoder/train_start_PWM.pl
+libexec/transdecoder/uri_unescape.pl