diff --git a/biology/samtools/Makefile b/biology/samtools/Makefile
index cbdfaa3b607b..64b06bff07e8 100644
--- a/biology/samtools/Makefile
+++ b/biology/samtools/Makefile
@@ -1,5 +1,5 @@
 PORTNAME=	samtools
-DISTVERSION=	1.15
+DISTVERSION=	1.16.1
 CATEGORIES=	biology
 
 MAINTAINER=	jwb@FreeBSD.org
diff --git a/biology/samtools/distinfo b/biology/samtools/distinfo
index 19439fbc44f5..d0c7b4cbb765 100644
--- a/biology/samtools/distinfo
+++ b/biology/samtools/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1645831653
-SHA256 (samtools-samtools-1.15_GH0.tar.gz) = 5109a17f221b36291e4bc3ad68a00c28515d4f20a16b4e23806055661ca0df31
-SIZE (samtools-samtools-1.15_GH0.tar.gz) = 4970205
+TIMESTAMP = 1672094602
+SHA256 (samtools-samtools-1.16.1_GH0.tar.gz) = 70a7f358088929272d8c22d6063290ac8420f5351fba237a99fe17851a45d8be
+SIZE (samtools-samtools-1.16.1_GH0.tar.gz) = 4992787
diff --git a/biology/samtools/pkg-plist b/biology/samtools/pkg-plist
index aafc040158e0..1d228254f042 100644
--- a/biology/samtools/pkg-plist
+++ b/biology/samtools/pkg-plist
@@ -48,6 +48,7 @@ man/man1/samtools-merge.1.gz
 man/man1/samtools-mpileup.1.gz
 man/man1/samtools-phase.1.gz
 man/man1/samtools-quickcheck.1.gz
+man/man1/samtools-reference.1.gz
 man/man1/samtools-reheader.1.gz
 man/man1/samtools-rmdup.1.gz
 man/man1/samtools-samples.1.gz